Endocrine Cell Culture (Handbooks in Practical Animal Cell Biology)
Cambridge University Press, 9/3/1998
EAN 9780521595636, ISBN10: 0521595630
Paperback, 168 pages, 22.9 x 15.2 x 1 cm
Language: English
This handy reference handbook describes the fundamental principles and procedures underlying the successful isolation of viable, functionally-intact cells from mammalian endocrine tissues, and their maintenance as primary cultures. The cell types selected for coverage illustrate the diversity of endocrine tissues from which cells have been isolated, and the range of procedures which have been devised to ensure the optimal survival and behaviour of each cell type under study. Particular emphasis has been placed on the provision of detailed protocols describing, step by step, the manipulations necessary to establish differentiated and responsive cultures. The chapters have been prepared by authors having direct practical experience of the cell type concerned, and the reader is therefore provided with first-hand accounts on the background to each procedure, the avoidance of potential problems and pitfalls, and the fundamental questions in endocrinology which may be addressed using each cell culture model.
List of contributors
Preface
Acknowledgements
Introduction Stephen Bidey
1. Parathyroid cells Ronal R. Macgregor
2. Ovarian granulosa Denis A. Magoffin
3. Anterior pituitary cells Seon H. Shin and John V. Milligan
4. Pancreatic ß-cells Leonard Best
5. Adrenocortical and adrenomedullary cells Matthias M. Weber, Christian Fottner and Dieter Engelhardt
6. Leydig cells Frank Chuzel, Hervé Lejeune and José Saez
7. Thyroid follicular cells Margaret C. Eggo
8. Hypothalamic cells Hilary E. Murray, Duncan McKenzie and Glenda E. Gillies
Index.
‘No one other book presents a collection of such contributions of the highest interest for all cellular and molecular biologists and we are sure that this booklet will meet a true great success, what we sincerely wish to it!’ R. Wegmann, Cellular and Molecular Biology
‘ … the book will be useful to graduate students and postdoctoral fellows in the laboratory having to prepare primary cultures of endocrine cells.’ Mario Vallejo, TEM
‘Crystal clear cell culture advice. You’ll want to wrap it in plastic and keep it on the lab bench … one of the most enjoyable and informative books that I have read in recent years.’ Elaine Hemers, British Society of Cell Biology
‘Crystal clear cell culture advice. You’ll want to wrap it in plastic and keep it on the lab bench.’ Gareth Cuttle, BSDB Newsletter