Methodologies for Metabolomics: Experimental Strategies and Techniques

Cambridge University Press, 3/21/2013
EAN 9780521765909, ISBN10: 0521765900

Hardcover, 640 pages, 25.3 x 17.7 x 3.5 cm
Language: English

Metabolomics, the global characterisation of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites, including nuclear magnetic resonance and mass spectrometry, are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples and diseases, ranging from in vivo metabolomics in humans and animals to in vitro analysis of tissue samples, cultured cells and biofluids.

List of contributors
Part I. Basic Methodological Strategies in Metabolomic Research
1. Exploring the human metabolome by nuclear magnetic resonance spectroscopy and mass spectroscopy
2. Methodological requirements for lipidomics research
3. Biological methods for metabolic research
Part II. Metabolomic Mass Spectrometry
Experimental Techniques and Bioinformatics
4. Considerations in sample preparation, collection, and extraction approaches applied in microbial, plant, and mammalian metabolic profiling
5. Mass spectrometry-based methodologies for single-cell metabolite detection and identification
6. Direct metabolomics from tissues and cells
laser ablation electrospray ionization for small molecule and lipid characterization
7. Bioinformatic approaches to processing and annotation of high-resolution mass spectrometry data
8. Approaches for natural product detection and structural elucidation using mass spectrometry with high mass accuracy
9. Metabolomics using ion mobility mass spectrometry
10. Metabolomics via biomedical mass spectrometry
from sampling to clinical applications
Part III. Metabolomics of Biofluids
Nuclear Magnetic Resonance Spectroscopy and Chemometrics
11. Analytical techniques in metabolomics integrating nuclear magnetic resonance spectroscopy and chromatography with mass spectrometry
12. Chemometric methods in nuclear magnetic resonance-based body fluid analysis
13. Nuclear magnetic resonance of cerebrospinal fluid
the neurometabolome
14. Nuclear magnetic resonance-based saliva metabolomics
15. Nuclear magnetic resonance methods for metabolomic investigation of amniotic fluid
16. Nuclear magnetic resonance analysis and genetic metabolic disease
17. Lipid profiling in health and disease
Part IV. Metabolomic Nuclear Magnetic Resonance Spectroscopy Techniques for Body Tissue Analysis
18. Magnetic resonance spectroscopy in investigating the cancer metabolome in preclinical model systems
19. Phospholipidomics by phosphorous nuclear magnetic resonance spectroscopy of tissue extracts
20. Carbon-13 nuclear magnetic resonance for analysis of metabolic pathways
21. Hyperpolarized nuclear magnetic resonance spectroscopy - a new method for metabolomic research
22. Metabolomic magnetic resonance spectroscopy of human tissues
comparison of in vivo and high-resolution magic angle spinning ex vivo techniques
23. Reproducible sample preparation and spectrum acquisition techniques for metabolic profiling of human tissues by proton high-resolution magic angle spinning nuclear magnetic resonance
24. Assignment strategies for nuclear magnetic resonances in metabolomics research
Index.